mOTUs: Profiling Taxonomic Composition, Transcriptional Activity and Strain Populations of Microbial Communities

The mOTU profiler, or mOTUs for short, is a software tool that enables the profiling of microbial communities in terms their taxonomic composition, relative abundance metabolically active members, and diversity strain populations. To this end, it maintains database single-copy phylogenetic marker gene sequences, which are used as reference to short read metagenomic metatranscriptomic reads mapped identification quantification taxa. Here, we describe most common use cases profiler two basic protocols. Additional supporting protocols provide information on its installation in-depth guidance adjusting settings increasing decreasing stringency with taxa detected quantified, well customizing output file format. Guidelines understanding results provided, along additional unique features, methodological details, development history tool. © 2021 Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Metagenomic 2: SNV Support Installing Profiling pipeline–step step 3: routine using advanced parameters 4: calling: